Phytochemical Study and Evaluation of the Biological Activity of Anorectic Plants Used in the Seno Province (Burkina Faso)

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Dramane Pare
Adama Hilou
Jotham Yhi-pênê N’DO
Sidonie Yabre
Nogma Ernest Sombie
Samson Guenne
Aristide Traoré
Odile Germaine Nacoulma


Background: In Africa plants have always been a good source of medicine for health care. Obesity is a pathology that is growing dramatically in developing countries. Anorectic plants are likely to cause a reduction of exaggerated weight gain. The aim of the study is to determine the phenolic compound content of five anorectic potential plants of Burkina Faso (Ceratotheca sesamoïdes, Gardenia erubescens, Brachystelma bingeri Raphionacme daronii and Vernonia kotschyana), to determine also their antioxidant potential and their acethylcholinesterase inhibitory capacity.

Place and Duration of Study: Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Research Institute for Health Sciences (IRSS).

Methodology: For the determination of the acute toxicity of the extracts a group of six (6) mice NMRI race were constituted for each plant extract. A dose of 3000 mg / kg of weight was administered to the animals. The methods of screening were used to detect secondary metabolites like tannins, steroids and terpen, flavonoids, coumarins. For the phenol content, the concentration of total phenolics, flavonoids and tannins were determined. The antioxidant property of the extracts was evaluated in vitro using 2,2-diphenyl-1-picrylhydrazyl acid (DPPH), 2,2-azino-bis (3-ethylbenzthiazoline-6-sufonic) (ABTS) and Ferric Reducing Antioxidant Power (FRAP). The acetylcholinesterase activity of the extracts 0.1 mg / ml was determined by a spectrometric assay method.

Results: Acute toxicity evaluated in NMRI mice showed that the methanolic extracts of five extracts show no toxicity. The coumarins and tannins were detected in all five species of plants. The polyphenol contents of Ceratotheca sesamoides gave the highest total phenolic compound content with 221.97 ± 1.206 mg EAG / g and also the best flavonoids content with 39.58 ± 0.068 mg EQ / g. Antioxidant tests show that Vernonia kotschyana Sch-Bip and Ceratotheca sesamoïdes Endl presented the best inhibitions of the DPPH radical with 82.63 ± 3.29% and 83.62 ± 2.12% at 100µg/ml. This activity is also better than that of quercetin which is a reference substance. For the reducing power of radical cation ABTS .+  the most active macerates of our extracts were obtained with Vernonia kotschyana (51,388 ± 0,133 mmol ET / g extract) and Ceratotheca sesamoides (50,748 ± 0,395 mmol ET / g extract). Ceratotheca sesamoides showed a best activity on reducing power of the ferric ion (7.03 ± 0.44 mmol EAA / g extract), this activity on ferric ion is superior to that of quercetin, which is a reference substance. Raphionacme daronii exhibited the greatest inhibition of acetylcholinesterase with a percentage inhibition of 53.542 ± 4.053 at 100 μg / ml.

Conclusion: The study demonstrated that anorexigenic plant extracts have a good antioxidant potential that is necessary for any weight-reducing activity. They also have an ability to inhibit acetylcholinesterase.

Anorectic plants, antioxidant, acetylcholinesterase activity

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How to Cite
Pare, D., Hilou, A., Yhi-pênê N’DO, J., Yabre, S., Sombie, N., Guenne, S., Traoré, A., & Nacoulma, O. (2019). Phytochemical Study and Evaluation of the Biological Activity of Anorectic Plants Used in the Seno Province (Burkina Faso). Journal of Scientific Research and Reports, 23(4), 1-13.
Original Research Article


Jäger AK, Hutchings A, van Staden J. Screening of Zulu medicinal plants for protaglandin-synthesis inhibitors. J of Ethnopharm. 1996;52:95-100.

seidell JC, Flegal KM. Assessing obesity: Classification and epidemi-ology. BMB. 1997;53:238–252.

Priyanka M, Pragyanshu K, Sneha J, Mahendra B, Kanthi KK, Kamlesh KB. Screening of six ayurvedic medicinal plants for anti-obesity potential: An investigation on bioactive constituents from Oroxylum indicum (L.) Kurz bark. Journal of Ethnopharmacology. 2017;197:138-146.

Van Heerden FR. Hoodia gordonii: A natural appetite suppressant. Journal of Ethnopharmacology. 2008;119:434-437.

Parveen K. Herbal fight for obesity. International Journal of Parmaceutical Research of Development. 2011;3(4):193-201.

Dimitri T. L’obésité: Découvertes récentes relatives aux mécanismes moléculaires à l’origine de nouvelles stratégies théra-peutiques. Université Henri Poincare - Nancy 1, Mémoire de thèse. 2010;1-79.

Halford J. Serotonin (5-HT) drugs: Effects on appetite expression and use for the treatment of obesity. Curr Drug Targets. 2005;6(2):201-13.

Antonucci F, Cangiano C, Cascino A, Ceci F, Del-Ben M, Laviano A, et al. Eating behavior and adherence to dietary prescriptions in obese adult subjects treated with 5-hydroxytryptophan. American Journal of Clinical Nutrition. 1992;56(5):863-7.

Igho O, Shao KH, Rachel P, Barbara W, Edzard E. The use of garcinia extract (Hydroxycitric Acid) as a weight loss supplement: A systematic review and meta-analysis of randomised clinical trials. Journal of Obesity. 2011;16-9.

Millogo R. L’homme, le climat, et les ressources alimentaires végétales en période de crise de subsistance au cours du 20ème siècle au Burkina Faso. Mémoire de thèse, Université de Ouagadougou. 2011;1-211. In French

MATD. Plan communal de developpement de. Dori 2009–2013, Rapport de la commune de Dori; MATD: Dori, Burkina Faso ; 2013. (In French)

Ligne directrice 423 de l’OCDE pour les essais de produits chimiques, Toxicité orale aiguë - Méthode par classe de toxicité aiguë; 2001;1-14. In French

Ciulei I. Pratical manuals on the industrial utilization of medicinal and aromatic plants: Methodology for analysis of vegetable drugs. Ministry of Chemical Industy, Bucharest. 1982;1-67.

Singleton LV, Orthofer R, Lamuela-Raventos RR. Analysis of total phenol and other oxydation substractes and antioxidants by mean of Folin- Ciocalteu reagent. Methode in Enzymology. 1999; 299;152-178.

Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardisation d’un extrait de Propolis et identification des principaux constituants. Journal de Pharmacie de Belgique. 1994;49:462-468. In French

Commission européenne. Procédures de prises en charge des céréales par les organismes d’intervention ainsi que les méthodes d’analyse pour la détermination de la qualité. Journal Officiel des Communautés Européennes. 2000;824:1-20. In French

Velazquez E, Tournier HA, Mordiyavich-Buschiazza P, Saavedra G, Schinnella GR. Antioxydant activity of Paraguayan plants extracts. Fitoterapia. 2003;74:91- 97.

Re R, Pelligrini N, Proteggente A, Yang M, Rice-Evans C. Antioxydant activity applying on improved ABTS radical cation decolourisation assay. Free Radical Biology, and Medicine. 1999;26:1231-1237.

Hinneburg I, Damien-Dordan HJ, Hiltunen R. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chemistry. 2006;97(1):122-129.

Lopez S, Batisda J, Viladomat F, Codina C. Acetylcholine inhibitory activity of some Amarayllidaceae alkaloids and Narcissus extracts. Life Sciences. 2002;71:251- 2529.

Hodge HC, Sterner JH. Determination of substances acute toxicity by DL50. American Industriel Hygien Association. 1943;10:1-93.

Ramadan G, Nadia M, El-Ghffar EAA. Modulatory effects of black v. Green tea aqueous extract on hyperglycaemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models. Br. J. Nutr. 2009;102:1611–1619.

Chen N, Bezzina R, Hinch E, Lewandowski PA, Cameron-Smith D, Mathai ML, et al. Green tea, black tea, and epigallocatechin modify body composition, improve glucose tolerance, and differentially alter metabolic gene expression in rats fed a high-fat diet. Nutr. Res. 2009;29:784–793.

Inngjerdingen KT, Meskini S, Austarheim I, Ballo N, Inngjerdingen M, Michaelsen TE, et al. Chemical and biological characteriza-tion of polysaccharides from wild and cultivated roots of Vernonia kotschyana. Journal of Ethnopharmacology. 2012; 139(2):350-8.

Nacoulma O. Plantes médicinales et pratiques médicales traditionnelles au Burkina Faso: Cas du plateau central. TOME II. Thèse d’Etat, Univ. Ouaga. 1996;1-260. In French

Kini F, Saba A, Ouedraogo S, Tingueri B, Sanou G, Guissou IP. Potentiel nutritionnel et thérapeutique de quelques espèces fruitières « sauvages » du Burkina Faso. Pharmacopée et Médecine Traditionnelle Africaines. 2008;15:32-35. In French

Kim JH, Kang SA, Han SM, Shim I. Comparison of the antiobesity effects of the protopanaxadiol- and protopanaxatriol-type saponins of red ginseng. Phytother Res. 2009;23(1):78-85.

Rodrigo R, Miranda A, Vergara L. Modulation of endogenous antioxidant system by wine polyphenols in human disease. Clin Chim Acta. 2011;412(5-6): 410-424.

Hasanein P, Fazeli F. Role of naringenin in protection against diabetic hyperalgesia and tactile allodynia in male wistar rats. J. Physiol. Biochem. 2014;70:997–1006.

Zang Y, Zhang L, Igarashi K, Yu C. The anti-obesity and anti-diabetic effects of kaempferol glycosides from unripe soybean leaves in high-fat-diet mice. Food Funct. 2001;6:834–841.

Sombié PAED. Évaluation du potentiel thérapeutique des galles de Guiera senegalensis J.F GMEL (Combretaceae) pour le traitement du diabète de type 2 et/ou de ses complications au Burkina Faso. Thèse doctorat, Université du Ouagadougou. 2013;1-200. In French

Bataille S, Ternaux J. Etude du rôle de l'acétylcholine et de l'acétylcholinestérase dans le développement des motoneurones spinaux et la protection contre un stress oxydatif. Thèse de doctorat, Université d'Aix-Marseille. 1999;1-155. In French

Mukherjee PKV, Kumar M, Mal PJH. Acetylcholinesterase inhibitors from plants. Phytomedicine. 2007;14:289–300.