Influence of Solvents and Solvent Mixtures on the Content of Polyphenols and Antioxidant Activity of Capsella bursa-pastoris (L.) Medik. Extracts
Emir Horozić *
Faculty of Technology, University of Tuzla, Urfeta Vejzagića 8, 75000 Tuzla, Bosnia and Herzegovina.
Edina Huseinović
Faculty of Science, University of Tuzla, Urfeta Vejzagića 4, 75 000 Tuzla, Bosnia and Herzegovina.
Lejla Mekić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Lamija Kolarević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Azra Hadžigrahić
Faculty of Medicine, University of Tuzla, Univerzitetska 1, 75 000 Tuzla, Bosnia and Herzegovina and University Clinical Center Tuzla, Ulica prof. dr. Ibre Pašića, 75 000 Tuzla, Bosnia and Herzegovina.
Enida Karić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Merima Ibišević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
*Author to whom correspondence should be addressed.
Abstract
Capsella bursa-pastoris (L.) Medik. (known as shepherd's purse) is a plant whose parts are used as medicine in herbal medicine. It is applicable as a medicine in the treatment of all forms of internal bleeding, for the treatment of hemorrhoids, excessive menstruation, but also for the usual stopping of nosebleeds. Through this research, the influence of organic solvents and their aqueous mixtures on the efficiency of polyphenol extraction and antioxidant activity was compared. The inhibition of free radicals was tested by the DPPH method, while the FRAP method was used to test the reduction potential. Analyzes have shown that water is the most effective solvent in the isolation of polyphenols from the aerial parts of shepherd's purse. Mixtures of organic solvents with water also showed high efficiency in the extraction of bioactive components, while the weakest results were obtained for extracts prepared in pure organic solvents.
Keywords: Shepherd's purse, TPC, FRAP, DPPH inhibition