Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

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Lincon Rafael da Silva
Marina Gabriela Marques
Paulo Henrique Pereira Costa Muniz
Thiago Alves Santos de Oliveira
Elizabeth Amélia Alves Duarte
Fabricio Rodrigues
Solange Xavier dos Santos
Daniel Diego Costa Carvalho


After long periods of storage, plant pathogen isolates lose their sporulation capacity. The objective of this study was to evaluate re-isolation methodologies for recovering sporulation of Pestalotiopsis grandis-urophylla isolates after subjection to a long period of storage. Isolates of P. grandis-urophylla were kept for 14 months on Petri dishes with PDA medium at 10°C. After this period, the isolate colonies showed reduced mycelial growth and no sporulation. The isolates were inoculated on healthy Eucalyptus grandis-urophylla leaves, and after ten days they were subjected to three re‑isolation methods: scraping of the lesions (S) removing of injured plant tissue fragments, followed by disinfestation (D) and without disinfestation (WD). Then, the purified isolates were evaluated for the recovery of its sporulation ability. The different methods for re-isolation resulted in the occurrence of differences among the isolates, showing that sporulation is an isolate-dependent feature. The three methods (S, WD and D) allowed the sporulation recovery of P. grandis-urophylla, even after these isolates have been subjected to 14 months.

Pathogenicity, mycelial growth, fungi storage.

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How to Cite
Silva, L. R. da, Marques, M. G., Muniz, P. H. P. C., Oliveira, T. A. S. de, Duarte, E. A. A., Rodrigues, F., Santos, S. X. dos, & Carvalho, D. D. C. (2021). Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage. Journal of Scientific Research and Reports, 27(1), 68-77.
Original Research Article


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