Distribution of msp1, msp2 and eba175 Allelic Family According to Hemoglobin Genotype and G6PD Type from Children with Uncomplicated Malaria in Banfora Heath District (Burkina Faso)
Salif Sombié *
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso and Unité de Recherche et de Formation Science de la vie et de la terre, Centre de Recherche en Sciences Biologiques et Alimentaires, Université Joseph KI-Zerbo, Burkina Faso.
Samuel Sindié Sermé
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Aïssatou Diawara
Department of Biology, Division of Science and Mathematics, New York University Abu Dhabi, Saadiyat Island, P.O.Box 41818, Abu Dhabi, UAE.
Mame Massar Dieng
Department of Biology, Division of Science and Mathematics, New York University Abu Dhabi, Saadiyat Island, P.O.Box 41818, Abu Dhabi, UAE.
Amidou Diarra
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Emilie S. Badoum
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso and Unité de Recherche et de Formation Science de la vie et de la terre, Centre de Recherche en Sciences Biologiques et Alimentaires, Université Joseph KI-Zerbo, Burkina Faso.
Sam Aboubacar Coulibaly
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Noelie Henry/Béré
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Wael Said Abdrabou
Department of Biology, Division of Science and Mathematics, New York University Abu Dhabi, Saadiyat Island, P.O.Box 41818, Abu Dhabi, UAE.
Aissata Barry
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Désiré Kargougou
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Alfred Sababeni Traoré
Unité de Recherche et de Formation Science de la vie et de la terre, Centre de Recherche en Sciences Biologiques et Alimentaires, Université Joseph KI-Zerbo, Burkina Faso.
Alfred B. Tiono
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
Sodiomon Bienvenu Sirima
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso and Groupe de Recherche Action en Santé (GRAS), Burkina Faso.
Youssef Idaghdour
Department of Biology, Division of Science and Mathematics, New York University Abu Dhabi, Saadiyat Island, P.O.Box 41818, Abu Dhabi, UAE.
Issiaka Soulama
Centre National de Recherche et de Formation sur le Paludisme, 01 BP 2208 Ouagadougou, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study aimed to evaluate the Plasmodium falciparum genetic diversity according to the host hemoglobin and G6PD genetic variants during the course of malaria in infected children aged from 2 to 10 years and living in endemic area in Burkina Faso.
Study Design: The study was designed as a longitudinal follow up conducted between May 2015 and February 2016 in Banfora health district, Burkina Faso.
Methodology: We included 136 subjects (73 males and 63 females; age range from 2-10 years). Blood thick and thin film was done by capillary blood. Venous blood was collected for DNA extraction. Malaria diagnosis was done by microscopy. Human and parasite DNA were extracted based on Qiagen kit procedure. Then, hemoglobin and G6PD were genotyped by RLFP-PCR while the msp1, msp2 and eba175 genes were typed by a nested PCR. All PCR products were analyzed by electrophoresis on a 1.5-2% agarose gel and alleles categorized according to the molecular weight.
Results: The prevalence of hemoglobin type was 19.11% for abnormal hemoglobin and 80.9% for normal hemoglobin carriage. The prevalence of G6PD type was 91.18% for normal and 8.82% for G6PD deficiency carriage, respectively. The prevalence of msp1 allelic families was 81.60%, 80.80% and 67.20% for k1, ro33 and mad20 respectively while for msp2 gene, fc27 and 3D7 allelic family the prevalence was 70.53% and 69.64% respectively. The eba175 allelic families’ distribution showed 77.31% and 40.21% for fcr3 and Camp respectively. There was no difference in multiplicity of infection (MOI) according to hemoglobin genotypes and G6PD types. We found that k1 was the predominant allelic family of msp1 in normal hemoglobin genotype (AA) and normal G6PD type. The mixed infection of eba175 was statistically higher in abnormal hemoglobin (p=0.04). There was no statistical difference between fcr3 and camp prevalence excepted in G6PD deficient type. The polymorphism results showed that the prevalence of 450 bp in fc27 was statistically significantly higher in normal hemoglobin variant carriers (AA) than abnormal hemoglobin carriers (p=2.10 -4)). However, the prevalence of 350 bp in fc27 was statistically higher in normal G6PD than deficient G6PD carriers (p=0.034).
Conclusion: Our result showed that the distribution of msp1 and eba75 polymorphism could be influenced by hemoglobin and G6PD variants. These results suggest that hemoglobin and G6PD could influence P. falciparum genetic diversity.
Keywords: Plasmodium falciparum, hemoglobin, G6PD, msp1, msp2, eba175, Burkina Faso.