Development and Characterization of Nano-Chitosan-Polyvinyl Alcohol-Glycerol -Chlorhexidine Films for Wound Healing Applications
Rosemol Jacob M.
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India and Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin, 682022, Kerala, India.
Amruth P.
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India and Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin, 682022, Kerala, India.
Preethy Treesa Paul
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India and Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin, 682022, Kerala, India.
Jean Mary Joy
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India and Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin, 682022, Kerala, India.
Visnuvinayagam S.
Microbiology, Fermentation and Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India.
Pavan Kumar Dara
Department of Biotechnology, SRM Institute of Science and Technology, Tamil Nadu, 603203, India.
R. Anandan
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India.
Suseela Mathew *
Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin, 682029, Kerala, India.
*Author to whom correspondence should be addressed.
Abstract
The process of wound healing is intricate and dynamic, requiring advanced medical care for a speedy recovery. Currently available semi-permeable wound dressings fall short in efficiently absorbing excess wound exudates to facilitate moist wound care, a crucial factor for promoting effective wound healing. Additionally, efforts have been made to enhance the antibacterial properties of wound dressings by incorporating antimicrobials into films. In this study, films were development using nanochitosan-polyvinyl alcohol-glycerol-chlorhexidine (NC-PVA-GLY-CLX) for applications in wound healing. The dressings were subjected to various characterizations, including UV-Visible spectrometry, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Thermogravimetric analysis, and X‑ray diffraction pattern. Furthermore, the resulting film dressings underwent evaluation for their bactericidal properties. The findings indicated that the incorporation of chlorhexidine into the films has positively influenced the fluid holding capacity and the active period of the dressing, creating a favourable environment for effective wound healing.
Keywords: Chlorhexidine, nanochitosan, chitosan nanoparticles, antimicrobial activity