Journal of Scientific Research and Reports

Ladybird beetles (Coccinella septempunctata L.) are vital allies in natural pest control, significantly benefiting crops like vegetables, fruits, and flowers by reducing the need for chemical pesticides. Understanding how temperature affects their life cycle is crucial for effective storage and conservation strategies.

Aim: This study investigated the impact of different temperatures on the survival and development of various life stages of C. septempunctata. Beetles were stored at four temperatures for 45 days.

Experiment Design: CRD (Completely Randomized Design).

Methodology: An experiment was conducted at the Department of Entomology RCA Udaipur to study the storage potential of predatory coccinellid species and the impact of cold storage on different stages. The study involved collecting different stages of coccinellids from a laboratory culture and storing them in a refrigerator at different temperatures and humidity levels for various periods. The data was then analyzed using statistical tools.

Results: Egg survival peaked at 12°C, followed by 10°C and 8°C, with the lowest survival at 6°C. Larval and pupal survival was highest at 10°C and 12°C, while both stages fared poorly at 6°C. While, male longevity was highest at 6°C, while females showed improved longevity at 8°C. Adult survival remained largely unaffected by temperature, though fecundity showed significant variation. After 15 days, no eggs survived at any storage temperature. The study found no survial of eggs and larvae after 30 days of storage at any temperature. The highest pupal survival was observed at 12°C and 10°C (1.20%), while no significant differences were observed between 8°C (0.60%) and 6°C (0.00%). The highest male longevity was recorded at 8°C (29.40 days), followed by 10°C (28.00 days). The highest female longevity was at 8°C (40.20 days), followed by 6°C (39.60 days). No significant differences were observed in adult survival across the different temperature treatments after 30 days of storage. Fecundity was statistically significant affected by storage temperature after 30 days, with the highest fecundity at 12°C (207.20 eggs) followed by 10°C (204.40 eggs).

Conclusion: The presnet findings indicated that a temperature range of 3–6°C offers optimal cold storage conditions, preserving beetle fitness without compromising survival or development. This present study provides valuable insights for enhancing biological control programs through improved storage strategies.