Evaluation of Different Extraction Techniques and Solvents on Yield of Polyphenolic Compounds from Pomegranate Peel (lat. Punica granatum)
Emina Ahmetović
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Aida Smajlagić *
Department of Chemistry, Faculty of Natural Sciences and Mathematics, University of Tuzla, Urfeta Vejzagića 4, 75 000 Tuzla, Bosnia and Herzegovina.
Samir Ćurić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Laura Divković
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Belkisa Djedović
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Ajla Džafić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Amina Hasikić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Sumea Haušić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Hedija Starčević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Merima Ibišević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Ermina Cilovic Kozarević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Lamija Kolarević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
*Author to whom correspondence should be addressed.
Abstract
In this study, polyphenolic compounds from pomegranate peel (Punica granatum) were extracted using different extraction methods. Three techniques were applied for polyphenol extraction: Soxhlet extraction, ultrasound-assisted extraction, and maceration. These methods varied in the time required for extraction and the yield of dry extract. For Soxhlet and ultrasound-assisted extraction, two solvents were used: methanol and ethanol. While maceration is simple and cost-effective, it was found to be the least efficient method for extraction. The removal of ethanol and methanol from the extracts was successfully achieved through evaporation, ensuring the purity of the extracts.The results obtained showed that Soxhlet extraction with methanol gave the highest yield of 33.5% compared to the ethanol solvent with 30.45%.Ultrasound-assisted extraction also yielded significant results, but the difference in yield was more pronounced depending on the solvent used. The goal of this study was to determine and present the efficiency of each extraction method. Further research will focus on assessing the antioxidant capacity of the extracted polyphenolic compounds.
Keywords: Extraction, polyphenolic compounds, Punica granatum, Soxhlet extraction, ultrasound-assisted extraction, maceration