Evaluation of Ex-vivo Cardioprotective and Anti-inflammatory Investigation of Bangladeshi Plants Extract
Mustafe Khalid Mohamed
Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh.
Anaytulla .
Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh.
Prawej Ansari *
Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh and Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh.
Md. Mizanur Rahman
Department of Pharmacy, International Islamic University Chittagong, 154/A, College Road, Chittagong-4203, Bangladesh.
Tarun Kumar Malik
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh.
Md. Mahmudul Hasan
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh.
Abul Kalam Azad
Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Dhaka-1229, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Background: The phytochemical screening of methanolic extract of Dalbergia stipulacea and Hymendictyon excelsum has indicated the presence of steroid, flavonoid and glycoside like anti-oxidative and cardioprotective compounds.
Methods: Since these compounds are of pharmacological interest, we got curious to check D. stipulacea and H. excelsum for their possible anti-inflammatory activity by HRBC (Human Red Blood Cell) membrane stabilization method, anti-arthritic activity by the inhibition of protein denaturation method and anti-thrombotic activity.
Results: The methanolic extracts of these plants exhibited notable anti-inflammatory activity and remarkable anti-arthritic, anti-thrombotic action. The maximum membrane stabilization of D. stipulacea and H. excelsum was found to be 72.33±2.52% and 69.33±2.52% at a dose of 1000 µg and that of protein denaturation was found to be 73.50±1.32% and 70.17±3.01% at the same dose, correspondingly. On the other hand when compared to Streptokinase for their anti-thrombotic activity, it was found that they were close to standard with p<0.0001 (from paired t-test), D. stipulacea and H. excelsum results 46.79±2.43% and 39.01±2.24%, respectively.
Conclusion: Therefore, our studies support the isolation and the use of active constituents from D. stipulacea and H. excelsum in treating inflammations and rheumatism as well as we should check for reason of its thrombolytic activity.
Keywords: Anti-inflammatory, anti-thrombotic, Dalbergia stipulacea, Hymendictyon excelsum, protein denaturation.