Optimization of Fibrinolytic Enzyme Production from Bacillus sp. IND6 in Solid State Fermentation
Mohammed A. Almalki
Biological Sciences Department, College of Science, King Faisal University, Saudi Arabia.
D. S. Deepa Dhas
Bioprocess Engineering Division, Smykon Biotech Pvt. Ltd., Nagercoil, Kanyakumari District, Tamilnadu, India.
Ponnuswamy Vijayaraghavan *
Bioprocess Engineering Division, Smykon Biotech Pvt. Ltd., Nagercoil, Kanyakumari District, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study aimed to screen and optimize Bacillus sp. IND6 for fibrinolytic enzymes production. The process parameters were optimized to enhance the production of fibrinolytic enzyme in solid state fermentation using wheat bran substrate.
Methodology: In this study fibrinolytic enzyme production was carried out using Bacillus sp. IND6 in solid-state fermentation. Wheat bran was used as the substrate. The process parameters were optimized using traditional one-variable-at-a-time approach.
Results: The significant increase in fibrinolytic enzyme production was occurred when this organism was grown up to 72 h (1310 ± 31.6 U/g), at pH 9.0 (1417.4 ± 73.2 U/g) with 90% moisture content (1441.2 ± 77.4 U/g). Among the carbon sources, starch significantly enhanced enzyme production (1713.8 ± 53.9 U/g).
Conclusion: Bacillus sp. IND6 could be the good source of fibrinolytic enzymes for various biotechnological applications.
Keywords: Fibrinolytic enzymes, Bacillus sp, solid state fermentation