Mutational Screening of the Negative Regulatory Region of Astrocyte Elevated Gene -1 Promoter in Hepatocellular Carcinoma Patients
Naghmeh Gholipour
Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Fatemeh Akbarian
Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Hamideh Khajeh
Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.
Sajjad Ghahhari
Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.
Nader Mansour Samaei *
Department of Human Genetic, Faculty of Medicine, Golestan university of Medical Sciences, Gorgan, Iran.
*Author to whom correspondence should be addressed.
Abstract
Objective: This study is aimed to assess the correlation between negative regulatory region of AEG-1 promoter and genetic susceptibility to hepatocellular carcinoma in Iranian population.
Methods: The negative regulatory region of human AEG-1 promoter was explored in 50 Iranian HCC patients. The PCR-sequencing method was used for investigating AEG-1 promoter polymorphisms.
Results: This analysis did not reveal the presence of any deletions, insertions, or mutations in this region ofAEG-1 promoter.
Conclusion: Mutations in the negative regulatory region of AEG-1 promoter have no significant relevance with hepatocellular carcinoma. Nevertheless, to our knowledge, this is the first study to investigate the mutations of the negative regulatory region of AEG-1 promoter in HCC patient. The role of AEG-1 promoter played in HCC needs to be further studied.
Keywords: Astrocyte elevated gene -1, promoter, hepatocellular carcinoma, mutation